Plasmid

Part:BBa_K5218003:Design

Designed by: Amy Fu, Juliette, Lichuan Chen, Xiaojuan Wang   Group: iGEM24_BGI-MammothEdu-South   (2024-08-18)


pS1300-AtC3H-GFP


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 234
    Illegal NheI site found at 552
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 406
    Illegal XhoI site found at 749
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 132
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1193
    Illegal SapI site found at 387


Design Notes

The RcTAL CDS fragment was cloned into pS1300-GFP vector via Gateway Cloning.


Source

The RcTAL CDS fragment was cloned into pS1300-GFP vector via Gateway Cloning.

References